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Viability and depletion of cell constituents of Methanospirillum hungatii GP1 during starvation

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/m80-154
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
Abstract
The viability of Methanospirillum hungatii GP1 cells dropped rapidly when starved in a pH 7.0 phosphate buffer under 100% N2 or 90% N2 – 10% H2 gas phase. The presence of H2 during starvation tests resulted in a more rapid and an ultimately greater reduction of the number of initially viable cells than in the presence of N2 only. The decrease in viability was observed concurrently with the decline of the ATP pool and the RNA content of the cells. The cell protein, carbohydrate, and amino acid pool also decreased. The cellular DNA content which increased initially under both gas phases subsequently decreased under N2–H2 gas phase. However, there was no net decline in the DNA content of the cells. The results indicate that M. hungatii would not survive prolonged periods of starvation.
Publication date
In
  • Canadian Journal of Microbiology 26 (1980): 887–892.
LanguageEnglish
NRC numberBREUIL1980
NRCC 18352
NPARC number9384833
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Record identifier1c0e7a33-c987-4817-a4cb-0b44093fc18e
Record created2009-07-10
Record modified2020-03-13
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