Pseudomonas aeruginosa D-arabinofuranose biosynthetic pathway and its role in type IV pilus assembly

  1. Get@NRC: Pseudomonas aeruginosa D-arabinofuranose biosynthetic pathway and its role in type IV pilus assembly (Opens in a new window)
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Journal titleJournal of Biological Chemistry
Pages2812828137; # of pages: 10
SubjectBioinformatics analysis; Biosynthetic pathway; Cell wall polymers; Chemical identity; d-Ribose; Epimerization; Glycosylated; Gram-negative bacteria; Knock outs; Mycobacterium smegmatis; Mycobacterium tuberculosis; Orthologues; P.aeruginosa; Pseudomonas aeruginosa; Therapeutic targets; Wild types; Biochemistry; Bioinformatics; Biosynthesis; Genes; Sugars; Bacteria; dextro arabinofuranose; pilin; sugar; type IVa pilin; unclassified drug; article; bacterial gene; bacterial strain; bacterium pilus; carbohydrate synthesis; cell motility; controlled study; epimerization; gene cluster; gene expression; mutant; nonhuman; polymerization; priority journal; protein assembly; protein glycosylation; Pseudomonas aeruginosa; wild type; Arabinose; Carbohydrate Epimerases; Fimbriae Proteins; Fimbriae, Bacterial; Glycosylation; Mutation; Mycobacterium tuberculosis; Pseudomonas aeruginosa; Corynebacterineae; Iva; Mycobacterium smegmatis; Mycobacterium tuberculosis; Negibacteria; Pila; Pseudomonas; Pseudomonas aeruginosa
AbstractPseudomonas aeruginosa strains PA7 and Pa5196 glycosylate their type IVa pilins with α1,5-linked D-arabinofuranose (D-Araf), a rare sugar configuration identical to that found in cell wall polymers of the Corynebacterineae. Despite this chemical identity, the pathway for biosynthesis of α1,5-D-Araf in Gram-negative bacteria is unknown. Bioinformatics analyses pointed to a cluster of seven P. aeruginosa genes, including homologues of the Mycobacterium tuberculosis genes Rv3806c, Rv3790, and Rv3791, required for synthesis of a polyprenyl-linked D-ribose precursor and its epimerization to D-Araf. Pa5196 mutants lacking the orthologues of those genes had non-arabinosylated pilins, poor twitching motility, and significantly fewer surface pili than the wild type even in a retraction-deficient (pilT) background. The Pa5196 pilus system assembled heterologous non-glycosylated pilins efficiently, demonstrating that it does not require post-translationally modified subunits. Together the data suggest that pilins of group IV strains need to be glycosylated for productive subunit-subunit interactions. A recombinant P. aeruginosa PAO1 strain co-expressing the genes for D-Araf biosynthesis, the pilin modification enzyme TfpW, and the acceptor PilA IV produced arabinosylated pili, confirming that the Pa5196 genes identified are both necessary and sufficient. A P. aeruginosa epimerase knock-out could be complemented with the corresponding Mycobacterium smegmatis gene, demonstrating conservation between the systems of the Corynebacterineae and Pseudomonas. This work describes a novel Gram-negative pathway for biosynthesis of D-Araf, a key therapeutic target in Corynebacterineae. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21271586
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Record identifier191688c3-8c5d-475d-896a-e1af67c003fc
Record created2014-03-24
Record modified2016-05-09
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