Enrichment and characterization of glycopeptide epitopes from complex mixtures

  1. Get@NRC: Enrichment and characterization of glycopeptide epitopes from complex mixtures (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/978-1-62703-589-7_11
AuthorSearch for: ; Search for: ; Search for:
Journal titleMethods in Molecular Biology
Pages181195; # of pages: 15
Subjectepitope; glycan; glycopeptide; glycoprotein; adaptive immunity; article; autoimmune disease; complex formation; electron transport; glycosylation; high performance liquid chromatography; immune system; innate immunity; mass spectrometry; priority journal; protein analysis; protein processing; tandem mass spectrometry
AbstractAntigen posttranslational modifications, including glycosylation, are recognized by the innate and adaptive arms of the immune system. Analytical approaches, including mass spectrometry and allied techniques, have allowed advances in the enrichment and identification of glyco-antigens, particularly T-cell epitopes. Similarly, major advances have been made in the identification, isolation, and detailed characterization of prokaryotic and eukaryotic glycoproteins and glycopeptides. In particular, peptide centric approaches are now capable of enriching low level glycopeptides from highly complex peptide mixtures. Similarly, advanced mass spectrometry methods allow identification of glycopeptides, characterization of glycans, and mapping of modification sites. Herein, we describe methods developed in our laboratory for the broad study of glycopeptides and illustrate how these approaches can be exploited to further our understanding of the identity and nature of glycopeptide epitopes in various diseases or auto immune disorders. © 2013 Springer Science+Business Media, LLC.
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AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics (HHT-TSH)
Peer reviewedYes
NPARC number21269650
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Record identifier1738bb2e-5799-4638-9be2-68fcd9eb5df7
Record created2013-12-13
Record modified2016-05-09
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