Recent progress in lentiviral vector mass production

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Journal titleBiochemical Engineering Journal
Pages362377; # of pages: 16
SubjectAnimal and insect cell culture engineering; bio; Biotechnology; Cell Culture; Large-scale production; Lentiviral vectors; methods; Scalable processes; therapeutic use; therapy; Viral vectors; Virus production
AbstractLentiviral vectors (LV) are promising tools for gene and cell therapy. They are presently used in several clinical trials as in vivo or ex vivo gene delivery vectors. However their mass production remains a challenge and might limit their potential therapeutic use. New robust and scalable processes are required for industrial production of these vectors. In this review, we focus on the assessment of current LV production methods and evaluate the most critical limitations with a focus on scalability. The key properties of LV are described and their inherent advantages and disadvantages discussed. A brief overview of the quantification methods generally used to characterize vector production is also provided as well as indications on downstream processing and basic regulatory aspects. The recent developments in the field including production in serum-free suspension cell cultures indicate that LV production is now amenable to industrial manufacturing using reliable large-scale processes. Crown Copyright -¬ 2009
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number52742
NPARC number12429590
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Record identifier13819680-125c-4e12-bb9d-36af326df085
Record created2009-10-26
Record modified2016-05-09
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